Learn how the Gateway ATR can yield spectroscopic information on low concentration biological samples.
Many biological samples are aqueous suspensions of large molecules and even whole organisms, such as bacteria. IR spectroscopy of aqueous samples can be challenging due to the strong absorbance of water. Horizontal multibounce ATR, often combined with temperature control, gives sufficient sensitivity to the biological components of a sample whilst also allowing for careful spectral subtraction of the water signal.
In this note we look at the analysis of yeasts and yeast protoplasts using the Gateway 6-reflection ATR. Spectra were acquired from aqueous suspensions of yeast cells and a comparison was made between spectra of whole cells and of protoplasts, which are an important precursor for new genetic hybrids.
Continue Reading in our PDF Below:
Related Application Notes
-
(Golden Gate ATR) AN21-10: Rapid Reaction Monitoring of UV Cured adhesives with the Specac High Temperature Golden Gate® ATR
-
(Golden Gate ATR) AN21-06: Study of Polymerization Kinetics of Dental Materials using the Golden Gate® ATR
-
(Golden Gate ATR) AN15-04: ATR Analysis of the Relative Concentrations of Active Pharmaceutical Ingredients using the Golden Gate® ATR
GET IN TOUCH