Reports on the use of Fourier transform infrared (FTIR) spectroscopy for analyzing biofluids (such as saliva, blood, and urine), tissues, and cells are growing rapidly. In this study, we evaluate the effectiveness of the Harrick ConcentratIR2™, a multi-reflection diamond ATR accessory, in analyzing minimal volumes of wild type mice urine.
Animal models, particularly mice, offer valuable insights in pre-clinical research due to their genetic and biological similarities to humans. By controlling genetics, environment, and diet, researchers can study large cohorts with reproducible conditions. Vibrational spectroscopy delivers molecular insights into the sample’s molecular structure, composition, and dynamics without requiring any tags or dyes.
The Harrick ConcentratIR2 ATR accessory enables quick and simple analysis of minimal volume biofluid samples, such as mice urine. It combines the high sensitivity of multiple-reflection ATR measurements with only microvolume sample requirements. In this case just 2 µL of sample was needed to obtain good spectra. The consistency of results affirms its effectiveness in capturing comparable data across different biological models.
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