Ensuring the integrity and consistency of peptide therapeutics at low concentrations is a growing analytical challenge in pharmaceutical development, particularly in the context of GLP-1 drug formulation, where protein-based active ingredients may need to be monitored at the µg/mL level.
Traditional transmission FTIR spectroscopy often lacks the sensitivity required at these low concentrations, with amide I and II bands, critical for confirming peptide structure, frequently obscured by noise or solvent background.
Clear resolution of amide bands in model pharmaceutical peptides Ziconotide, Calcitonin, and Leuprolide Acetate at concentrations relevant to early-stage formulation and quality control was proven to be possible using multiple reflection ATR on samples of only millilitre volume.
The Harrick ConcentratIR2™ ATR accessory combines the high sensitivity of multiple-reflection ATR measurements with only microvolume sample requirements. In this case just 10 µL of sample was needed to obtain good spectra. The consistency of results affirms its effectiveness in capturing comparable data across different biological models.
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